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. 2015 Mar 6;11(3):e1005036. doi: 10.1371/journal.pgen.1005036

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© 2015 Orgil et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 8 — A. Strains YOG3021 (SCC3-6HA scc3-6), YOG3024 (scc3-Y371A-6HA scc3-6) and YOG3025 (scc3-K372A-6HA scc3-6), were grown at permissive temperature (23°C) and arrested at G1 using α-factor. The cells were shifted to restrictive temperature (35.5°C) and re-arrested in the G2/M phase using nocodazole. Nuclei were spread and the bulk DNA was stained with DAPI. The morphology of the rDNA-loop of 100 nuclei of each strain was scored (n = 3). B. Representative photomicrographs of wild-type rDNA loops (left panel) and condensation defects at the rDNA locus. Arrow indicates the rDNA region.