Fig 4. Effect of K252a on H₂S-induced suppression in formaldehyde-mediated oxidative stress in PC12 cells.

PC12 cells were preincubated with K252a (10 nM) for 30 min before pretreatment with NaHS (200 μM) for 30 min, and then cotreated with formaldehyde (FA, 120 μM) for 24 h. (A) The generation of intracellular ROS was measured by NBT reduction assay as described in “Materials and methods.”. (B) The level of MDA in PC12 cells was measured by MDA Elisa kits. (C) The level of 4-HNE in PC12 cells was measured by 4-HNE Elisa kits. Results were expressed as the mean ± S.E.M. of three independent experiments. *P < 0.05, **P < 0.01, versus control group; ^## P <0.01, versus FA-treated alone group; ^&& P < 0.01, versus cotreated with NaHS and FA group.