Table 1. Estimates of alpha diversity for the fresh, −80°C frozen and snap frozen samples.

Data set	Fresh	Frozen −80°C	Snap frozen	p-value
Chao1 richness estimate	846	964	878	0.905
Shannon index	5.75	5.97	5.87	0.662
Simpson's diversity index	0.95	0.96	0.95	0.754
Number of observed species	813	927	816	0.865