Fig 3. Psammaplin A, but not vitamin C, reduces DNA methylation at 72 h post-activation.

ICSI embryos and cloned embryos non-treated (NT) and treated with 100 μM vitamin C (VitC), 10 μM psammaplin A (PsA) or the combination of both (VitC-PsA) during 16 h were immunostained for 5meC and DNA detection. A) Representative images of DNA and 5meC staining. Scale bar = 20 μm. B) Average intensity of 5meC/DNA signal ratio (+SEM) relative to ICSI embryos.