. 2015 Mar 6;10(3):e0120033. doi: 10.1371/journal.pone.0120033

Table 1. Effect of different durations of 100 μM VitC treatment on the in vitro development and quality of cloned mouse embryos.

			No. activated embryos developed to (%)				Mean cell number (±SEM) 96 h p.a.
Group	No. reconstructed	No. activated (%)	Two cell	Morula	Blastocyst	No. blastocysts	ICM	TE	Total
NT	186	176 (94.6) ^a	143 (81.3) ^a ^, ^b	111 (63.1) ^a ^, ^b	47 (26.7) ^a	46	10.7±0.7 ^a	24.9±1.5 ^a	35.6±2.1 ^a
VitC 8–9 h	206	200 (97.1) ^a	159 (79.5) ^a	112 (56.0) ^a	71 (35.5) ^a ^, ^b	39	12.8±0.9 ^a ^, ^b	26.2±1.7 ^a ^, ^b	39.0±2.4 ^a ^, ^b
VitC 16 h	219	209 (95.4) ^a	188 (90.0) ^c	152 (72.7) ^b ^, ^c	89 (42.6) ^b ^, ^c	45	15.0±0.7 ^b	31.5±1.3 ^a ^, ^b	46.4±1.8 ^b
VitC 24 h	218	208 (95.4) ^a	184 (88.5) ^b ^, ^c	149 (71.6) ^b ^, ^c	92 (44.2) ^b ^, ^c	43	14.3±0.7 ^b	31.4±1.5 ^a ^, ^b	45.7±2.1 ^a ^, ^b
VitC 120 h	214	207 (96.7) ^a	186 (89.9) ^c	159 (76.8) ^c	95 (45.9) ^c	47	15.3±0.8 ^b	32.8±1.5 ^b	43.1±2.1 ^b

NT: non-treated cloned embryos; p.a.: post-activation

^a-c Values with different superscripts differ significantly within the same column (p < 0.05; Chi-square test for embryonic development and Kruskall-Wallis for blastocysts cell numbers).