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. 2015 Mar 5;25(2):173–177. doi: 10.1097/CMR.0000000000000141

Fig. 1.

Fig. 1

Methylation levels of LINE-1 and repetitive DNA sequences in melanoma patients. (a, b) Methylation levels of four LINE-1 CpGs (positions 331 to 305, GenBank accession X58075) obtained using quantitative bisulfite pyrosequencing. (c, d) Methylation levels of 22 436 CpGs mapped in repetitive DNA elements measured using the HM450K platform (Illumina). (a) Melanoma patients exhibited significantly higher overall and specific LINE-1 methylations levels compared with controls (the Mann–Whitney test). (b) The methylation levels at LINE-1 CpGs positions 2 and 4 were significantly different among the groups of melanoma patients (the Kruskal–Wallis with Dunn’s multiple comparison post-test). (c) Repetitive DNA elements did not show significant difference in methylation levels between melanoma patients and controls (the Mann–Whitney test). (d) Familial melanoma patients carrying CDKN2A mutations showed a hypermethylated pattern of repetitive DNA elements compared with melanoma patients without mutations (Kruskal–Wallis with Dunn’s multiple comparison post-test). *P<0.05.