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. 2015 Mar 6;10(3):e0119177. doi: 10.1371/journal.pone.0119177

Fig 5. Physiological role of Fam20a in chondrocytes.

Fig 5

(A) Expression of Fam20a was knocked down using siRNA (siFam) in primary murine articular chondrocytes. The effect of the siRNA was evaluated by comparison with primary murine chondrocytes transfected with scrambled-sequence siRNA as a negative control (NC). Expression of Fam20a was analyzed by quantitative real-time PCR. Expression of GAPDH was used as an internal control. ***P < 0.001. (B) Analysis of osteoarthritis-related genes in Fam20a knocked-down cells. Expression of Col2a1 and aggrecan was measured as major proteins comprising the extracellular matrix of articular chondrocytes. Expression of ADAMTS5 and Mmp-13 was measured as major enzymes that degrade the extracellular matrix. Expression of GAPDH was used as an internal control. A significant decrease of Col2a1 expression was observed in Fam20a knocked-down chondrocytes. *P < 0.05.