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. Author manuscript; available in PMC: 2015 Mar 6.
Published in final edited form as: DNA Repair (Amst). 2010 Apr 10;9(6):718–726. doi: 10.1016/j.dnarep.2010.02.013

Figure 1. Phosphorylation of Slx4 during repair of a HO–induced DSB by SSA.

Figure 1

(a) Schematic diagram of SSA strains used in this study. (b) Strains GTY14 (EAY1141 Slx4-13Myc) and GTY27 (YMV80 Slx4-13Myc) were grown in YEP-lactate medium overnight, and HO expression was induced by the addition of galactose. Aliquots were taken at the indicated times after addition of galactose and analysed by Western blotting for Slx4 and Rad53. (c) Western blot analysis of Slx4 and Rad53 hyperphosphorylation in YMV80 Slx4-13Myc strains in which RAD1 (GTY42) or SAW1 (GTY44) were deleted. (d) Western blot analysis of Slx4 and Rad53 hyperphosphorylation in YMV80 Slx4-13Myc strains in which MEC1 (GTY37), TEL1 (GTY38), or both MEC1 and TEL1 (GTY39) were deleted.