Figure 1.

Real-time RT-PCR analysis of mRNA transcripts of HGrC1. HGrC1 was stimulated for 48 hours with 5 IU/mL FSH, 2 μg/mL GDF9, or both. Combined effects on upregulation were seen in HMGCS and CYP51A1. The data are presented as the mean ± SD of 3 independent replicates. Different letters show significant difference (P < .05). RT-PCR indicates reverse transcription polymerase chain reaction; mRNA, messenger RNA; FSH, follicle-stimulating hormone; HGrC1, human immortalized nonluteinized granulosa cell line; GDF9, growth differentiation factor 9; HMGCS, 3-hydroxy-3-methylglutanyl-CoA synthase 1; CYP51A1, cytochrome P450, family 51, subfamily A, polypeptide 1; SD, standard deviation.