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. 2015 Mar;22(3):377–384. doi: 10.1177/1933719114529375

Figure 2.

Figure 2.

Immunoblotting for CYP51A1 and HMGCS. Representative images of immunoblotting against CYP51A1 (57 kDa), HMGCS (57 kDa), and β-actin (42 kDa) expressed by HGrC1. CYP51A1 was significantly induced by stimulation of cells with GDF9, FSH, or both for 48 hours in HGrC1 cells (upper panel). HMGCS was similarly induced by stimulation of cells with GDF9 or GDF-9 + FSH for 48 hours in HGrC1 cells (middle panel). HMGCS indicates 3-hydroxy-3-methylglutanyl-CoA synthase 1; CYP51A1, cytochrome P450, family 51, subfamily A, polypeptide 1; HGrC1, human immortalized nonluteinized granulosa cell line; GDF9, growth differentiation factor 9; FSH, follicle-stimulating hormone.