Table 1.
Primer Sets Used for Real-Time RT-PCR.
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
| HMGCS1 | AAGTCCAGGCCAGCAGTGA | ATATTCACAGCTCCTGAATGTACCA |
| SC4MOL | GCATGGGTGACCATTCGTTTATTAG | CCCACCATGTAAATGTTGAAGCA |
| FDFT1 | AGAATATTGACTTGGCCGTGCAG | GGCAGCCAAAGTGGCAATG |
| DHCR7 | GGAGCTCCACAGCCATGTGA | TTCAGGTACCAGGTTTCGTTCCA |
| HMGCR | CTTGCTTGCCGAGCCTAATGA | ACTAGGCACAGTTCTAGGGCCATTC |
| SQLE | TTGTGATGGGAGTTCAGTACAAGGA | GCCCATCTGCAACAACAGTCA |
| MVK | GGAGCTAATTAACAAGTGGGCCTTC | GTGTTGGTCAGCAGGATCTGGA |
| LSS | GAGGGTTCGCCACCTATGAGA | GAAATACTTAAGCGCCTGCATCAC |
| CYP51A1 | CTACAGTCGCCTGACAACAC | CCACTTTCTCCCCAACTCTC |
| NSDHL | CCTATGCCATGAAACCCATTGAC | ACGAACTTCATCTTGCCGTTCC |
| FDPS | GCATGTATCTACCGCCTGCTGA | TCCAGGGTCTGCCCAATCTC |
Abbreviations: HMGCS1, 3-hydroxy-3-methylglutanyl-CoA synthase 1; SC4MOL, sterol-C4-methyl oxidase-like; FDFT1, farnesyl-diphosphate farnesyltransferase 1; DHCR7, 7-dehydrocholesterol reductase; HMGCR, 3-hydroxyl-3-methylglutanyl-CoA reductase; SQLE, squalene epoxidase; MVK, mevalonate kinase; LSS, lanosterol synthase; CYP51A1, cytochrome P450, family 51, subfamily A, polypeptide 1; NSDHL, NAD(P)-dependent steroid dehydrogenase-like; FDPS, farnesyl diphosphate synthase; RT-PCR, reverse transcription polymerase chain reaction; NAD(P), nicotinamide adenine dinucleotide phosphate.