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SDS-PAGE analysis non-reducing SDS-PAGE (A) and reducing SDS-PAGE (B) of produced monoclonal antibody. In reduced form (2 and 3), two bands were seen in 50 and 25 kDa but in non-reducing SDS-PAGE condition, only one band was seen in about 150 kDa. The SDS-PAGE gel lane 1 contains a band at 150 kDa is in non-reducing condition, in lane 2, 3 contain two band at 25 and 50 kDa in reducing condition and lane 4 produced antibody before purification.
