Table 3.
Primers used in this study
| Primer Name | Primer Sequence 5′ − 3′ |
|---|---|
| GST-VieS-C F | tcactgtgcatatgactgagcagctacgttggttgacgg |
| GST-VieS-C R | gagcgagtcggatcctcagagataacgactgagtactttgcgc |
| GST-VieS-C T2916C F | gttgattactgactgccacatgccacatcttgatg |
| GST-VieS-C T2916C R | catcaagatgtggcatgtggcagtcagtaatcaac |
| VieB F | tcactgtgcatatggctgtacctacttttgctgaattaaaag |
| VieB R | gagcgagtcggatccttacgcctcaactgattcgcttcgc |
| VieB D62A F | gatttgatatttttatttgcgcttacaacttcggtaaggggtt |
| VieB D62A R | aaccccttaccgaagttgtaagcgcaaataaaaatatcaaatc |
| VieB D62E F | gatttgatatttttatttgcgagtacaacttcggtaaggggtt |
| VieB D62E R | aaccccttaccgaagttgtactcgcaaataaaaatatcaaatc |
| MBP-VieS-C F | CGCGGATCCTTACGCAGCTCCGAACAAG |
| MBP-VieS-C R | ACGCGTCGACTTATTCGCTCTGATACTGATG |
| MBP-VieS-C D1018A F | TATGGCAATTGGTAATCAACAAATCATACTGCTCAGGATGTTGCGAGAGCTTTT |
| MBP-VieS-C D1018A R | CGAAAAGCTCTCGCAACATCCTGAGCAGTATGATTTGTTGATTACCAATTGCCA |
All primers are listed 5′ to 3′. Italics indicate restriction enzymes used for cloning.