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. 2015 Feb 27;15:65. doi: 10.1186/s12870-015-0454-0

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© Naoumkina et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sources of variability in RNA-seq data. (A) Principal component analysis of RNA-seq samples from developing fibers (at 8 DPA) of Li ₁, Li ₂ and WT NILs. F: field grown plants; GH: greenhouse grown plants. (B) Proportion of the transcriptional variance explained by each variance component. L: near-isogenic lines, Li ₁, Li ₂ and WT; E: environmental factors, greenhouse and field; BR: biological replicates; and R: residual.