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. 2015 Mar 1;13:76. doi: 10.1186/s12967-015-0444-x

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© Moreira et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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AKT activation in ischemic hearts. A: Illustrative representation of all experimental groups. “IR”, Ischemia-reperfusion. “Pre”, SC79 administered before ischemia. “Post”, SC79 administered at reperfusion. “Both”, SC79 administered before ischemia and at reperfusion. “Low” indicates 100nM SC79 and “High” indicates 300nM SC79. B: AKT phosphorylation in normoxic-perfused (N) or ischemic hearts (IR) treated with DMSO or SC79. C: Total AKT expression; D: Correlation between AKT phosphorylation and GSK-3β phosphorylation. E: Correlation between AKT phosphorylation and Ribosomal-S6 phosphorylation. The Pearson correlation test was used to analyze the correlations shown in panels D and E. Number of animals analyzed per group is displayed within the bars. Western blot bands displayed in panels D and E are disposed in the same group order as in panels B and C. Data are shown as mean ± SE. #, p < 0.05 vs. IR-Control; &, p < 0.05 vs. all other groups.