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. Author manuscript; available in PMC: 2015 Mar 7.
Published in final edited form as: Methods Enzymol. 2014;536:47–52. doi: 10.1016/B978-0-12-420070-8.00004-0
Component Final concentration Stock Amount
Tris–HCl, pH 8.0 25 mM 1M 2.5 ml
NaCl 300 mM 5M 6 ml
Glycerol 10% 100% 10 ml
EDTA 1 mM 500 mM 0.2 ml
PMSF 1 mM 100 mM 1 ml
Add water to 100 ml