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. Author manuscript; available in PMC: 2015 Mar 7.
Published in final edited form as: Cancer Res. 2014 Feb 24;74(8):2258–2269. doi: 10.1158/0008-5472.CAN-13-2459

Figure 2.

Figure 2

Expression of NF-κB–dependent genes in paclitaxel-resistant ovarian cancer cells. A, total protein was isolated from SKOV3, OVCAR3, SKOV3TR, and OVCAR3TR cells. Levels of RSF1, CFLAR, BCL2, BCL2L1, and PTGS2 protein were analyzed by Western blotting using specific antibodies. Expression levels were normalized with β-actin. Data are representative of three different experiments. B, total RNA was isolated from SKOV3, OVCAR3, SKOV3TR, and OVCAR3TR cells. mRNA expression of RSF1, CFLAR, BCL2, BCL2L1, and PTGS2 was analyzed by real-time RT-PCR. Data were normalized to GAPDH mRNA expression. The data are representative of three different experiments and are shown as the mean ± SD. *, P < 0.05 compared with the parental cells group.