Figure 7.

Involvement of hSNF2H in interaction between RSF1 and NF-κB. A, an anti-p65 antibody was used to pull-down associated proteins from a protein lysate. Samples were analyzed by Western blotting using anti-RSF1, hSNF2H, NF-κB p65 subunit, and CBP-specific antibodies. Lane 1, immunoprecipitation (IP) with indicated antibody in RSF1-noninduced SKOV3 cells; lane 2, immunoprecipitation with indicated antibody in RSF1-induced SKOV3 cells; lane 3, immunoprecipitation with control IgG in RSF1-induced SKOV3 cells; lane 4, no cells with control IgG; lane 5, immunoprecipitation with indicated antibody in RSF1-overexpressing OVCAR3 cells; and lane 6, immunoprecipitation with indicated antibody in A2780 cells, which barely express RSF1. B and C, RSF1-induced SKOV3, SKOV3^TR, and OVCAR3^TR cells were transfected with hSNF2H siRNA (50 nmol/L) or control siRNA, and protein lysate was used to perform an immunoprecipitation with an anti–NF-κB antibody. Samples were analyzed by Western blotting using anti-RSF1, hSNF2H, NF-κB, and CBP-specific antibodies. Data are representative of three different experiments.