Fig. 2.

Strategy of proteomic analysis for identifying miR-145 targets. miR-145 mimic was transfected into MiaPaCa-2 cells that were labeled with heavy amino acids while scrambled RNAs were transfected into unlabeled cells. Proteins from both groups of cells were extracted 48 hours after transfection and fractionated through four methods: in-gel digestion, strong cation exchange chromatography, off-gel peptide and protein pI-based separations. Fractionated peptides were then analyzed on an LTQ-Orbitrap Velos mass spectrometer.