Figure 6. Immunomodulatory mixed leukocyte reaction experiment media concentrations of (A & D) IFN-γ, (B & E) PGE2 and (C & F) TGF-β1 for control MHC-mismatched MLRs and for MHC-mismatched MLRs cultured with matched or mismatched MSCs or iPSCs.
Media from experiments using responder leukocyte concentrations of 4 × 105 cells (A–C) and 8 × 105 cells (D–F) were examined based on the significant immunomodulatory results found at these concentrations as shown in Figures 4 & 5. Bars represent mean ± SD from a total of three pooled samples of each cell type from each MLR experiment. Superscript letters indicate significant differences between groups by ANOVA, followed by a LSD multiple comparisons test; p ≤ 0.05.
iPSC: Induced pluripotent stem cell; MEF: Mouse embryonic fibrobast; MHC-M: MHC-matched; MHC-MM: MHC-mismatched;
MLR: Mixed leukocyte reaction; MSC: Mesenchymal stromal cell; PGE2: Prostaglandin E2.