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. 2014 Nov 26;3(2):114–117. doi: 10.1002/ccr3.168

Table 1.

Primer sequences of the coding exons and flanking intron sequences with appropriate PCR product length and annealing temperatures

Exon Sequence Tm (C) Size (bp)
X2-F AAGTTGCCGATGGATGTTGT 60.38 168
X2-R tttgaagaagaaaatgagcacttg 59.93
X3-F gtttcctcagagtggccaaa 60.23 336
X3-R gtcaccagagggaccagatg 60.53
X4-F ctggaagcgcaaatgttaga 59.06 199
X4-R acactgtctgtcagcccctc 60.32
X5-F gaatggcctgaagtgtggtt 59.97 227
X5-R ctgtgggtgtcaggcgtc 61.38
X6-F gtccggtggacgctgaag 62.89 229
X6-R cctggggtgatgcctctg 62.71
X7-F gcagtgctgagtccctgtg 60.63 203
X7-R acgtgacgtttaatccagcc 60.00
X8-F ctccacttccttatgagccg 59.83 251
X8-R tgaatgcaccaagactgagc 59.99
X9-F ttggaattcgacttcttcgac 59.30 192
X9-R caccaagggagggctagg 60.60
X10-F aaaaggcagaggtttcagca 59.99 324
X10-R agagcaccacatgtctggg 59.68
X11-F gagggagctttggtctcctg 61.30 277
X11-R ccactcacctccccagtg 60.08
X12-F tggccctggtgaagtaacac 60.95 457
X12-R TGAGAGAGGCAGGAAGAGGA 60.21

Exon 1 is noncoding. The same primers were used for direct sequencing of PCR products.