Table 1.
Exon | Sequence | Tm (C) | Size (bp) |
---|---|---|---|
X2-F | AAGTTGCCGATGGATGTTGT | 60.38 | 168 |
X2-R | tttgaagaagaaaatgagcacttg | 59.93 | |
X3-F | gtttcctcagagtggccaaa | 60.23 | 336 |
X3-R | gtcaccagagggaccagatg | 60.53 | |
X4-F | ctggaagcgcaaatgttaga | 59.06 | 199 |
X4-R | acactgtctgtcagcccctc | 60.32 | |
X5-F | gaatggcctgaagtgtggtt | 59.97 | 227 |
X5-R | ctgtgggtgtcaggcgtc | 61.38 | |
X6-F | gtccggtggacgctgaag | 62.89 | 229 |
X6-R | cctggggtgatgcctctg | 62.71 | |
X7-F | gcagtgctgagtccctgtg | 60.63 | 203 |
X7-R | acgtgacgtttaatccagcc | 60.00 | |
X8-F | ctccacttccttatgagccg | 59.83 | 251 |
X8-R | tgaatgcaccaagactgagc | 59.99 | |
X9-F | ttggaattcgacttcttcgac | 59.30 | 192 |
X9-R | caccaagggagggctagg | 60.60 | |
X10-F | aaaaggcagaggtttcagca | 59.99 | 324 |
X10-R | agagcaccacatgtctggg | 59.68 | |
X11-F | gagggagctttggtctcctg | 61.30 | 277 |
X11-R | ccactcacctccccagtg | 60.08 | |
X12-F | tggccctggtgaagtaacac | 60.95 | 457 |
X12-R | TGAGAGAGGCAGGAAGAGGA | 60.21 |
Exon 1 is noncoding. The same primers were used for direct sequencing of PCR products.