Fig. 3.
Size exclusion chromatography profile of guinea pig lens extract treated with and without αA66-80 peptide. WS guinea pig lens extract (WS 200 μg) was incubated in the presence of αA66-80 peptide (0, 25, 50, 75, 100 μg, in 100 μl) overnight (16 h) at 37°C. The samples were centrifuged at 10,000 × g for 10 min to remove precipitate, if any, and the supernatant was injected into a TSKG-5000PWXL column (7.8 mm × 30 cm). The elution of protein was monitored using a 280-nm absorbance detector. The representative profiles show that αA66-80 peptide selectively targets α-crystallin in a concentration-depended manner. The inset shows the amount of soluble crystallins recovered prior to chromatographic analysis. The amount of protein recovered with scrambled αA66-80 peptide (100 μg) as a control is shown in the gray color bar. (n=3).