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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Exp Eye Res. 2015 Jan 15;132:52–58. doi: 10.1016/j.exer.2015.01.012

Fig. 2.

Fig. 2

To examine reversal, cytoplasmic calcium concentration was measured in cells exposed to hyposmotic solution (200 mOsm) for 2 min then returned to control (isosmotic) solution (Panel A). To examine dependence on extracellular calcium cytoplasmic calcium concentration was measured in cells exposed to calcium-free hyposmotic solution (200 mOsm) (Panel B). Baseline calcium concentration was first measured for 5 min in control isosmotic solution (Iso) then calcium-free hyposmotic solution (200 mOsm) was introduced and the measurement continued for a further 30 min. Data from 15–30 individual cells were averaged and considered as n=1. Results are means ± SE of 5 independent experiments.