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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Exp Eye Res. 2015 Jan 15;132:52–58. doi: 10.1016/j.exer.2015.01.012

Fig. 3.

Fig. 3

The effect of GAP 27, a connexin inhibitor, on the cytoplasmic calcium response to hyposmotic solution. Panel A: cells were preincubated in isosmotic solution for 60 min with GAP 27 (200 µM) then baseline cytoplasmic calcium concentration was measured for 5 min before the cells were exposed to hyposmotic solution (200 mOsm) in the continued presence of GAP 27. Data from 15–30 individual cells were averaged and considered as n=1. The values are the mean ± SE of results from 3–5 independent experiments. *** indicates a significant difference (p< 0.001) between hyposmotic solution alone and hyposmotic solution plus GAP 27. Panel B shows reversal of the calcium response by GAP 27. Cells were exposed to hyposmotic solution (Hypo), causing an increase in cytoplasmic calcium concentration. GAP 27 (200 µM) was introduced 2 min later and calcium was monitored continuously in the hyposmotic GAP 27-containing solution. Data from 15–30 individual cells were averaged and considered as n=1. Results are means ±SE of 3–5 independent experiments. *** indicates a significant difference (p< 0.001) between hyposmotic solution alone and hyposmotic solution plus GAP 27.