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. Author manuscript; available in PMC: 2015 Mar 7.
Published in final edited form as: Otolaryngol Head Neck Surg. 2011 Oct 26;146(1):46–52. doi: 10.1177/0194599811425141

Figure 2.

Figure 2

Histological sections stained with H&E and with Alcian Blue for detection of GAG. (a) H&E staining for pre-implant construct sectioned at 40μm. (b) H&E stain of post-30-day implant sectioned at 5μm. (c) Alcian Blue stain of post-30-day implant sectioned at 5μm. (d) H&E stain of post-60-day implant sectioned at 5μm. (e) Alcian Blue stain of post-60-day implant sectioned at 5μm. H&E stain of the neocartilage 30 days post-implantation shows homogeneous distribution of chondrocytes within a cell matrix. At 60 days, the implant exhibits architecture more similar to native septal cartilage.13 A moderate to substantial amount of GAG is observed with Alcian Blue staining at 30 and 60 days.