Table 1.
Characteristics of the anti-dsDNA antibody assays used in this study.
| Characteristic | QUANTA Flash dsDNA | QUANTA Lite dsDNA SC | BioPlex 2200 dsDNA | ImmuLisa dsDNA | NOVA Lite dsDNA |
|---|---|---|---|---|---|
| Manufacturer | Inova Diagnostics | Inova Diagnostics | Bio-Rad | Immco Diagnostics | Inova Diagnostics |
| Technology | CIA | ELISA | ALBIA | ELISA | CLIFT |
| Assay timer | 30 min | 90 min | 45 min | 90 min | 60 min |
| Detection | Quantitative | Quantitative | Quantitative | Semi-quantitative | Semi-quantitative |
| Analytical measuring range | 9.8–666.9 IU/mL | 12.3–1000 IU/mL | 1–300 IU/mL | 13.6–450 IU/mL | N/A |
| Cut-off value (ranges) | 9.8–35 negative 35–45 equivocal >45 positive |
12.3–30 negative 30–75 equivocal >75 positive |
1–5 negative 5–9 indeterminate >9 positive |
13.6–50 negative 50–60 borderline >60 positive |
N/A |
| Cut-off value applied | ≥35 IU/mL | ≥30 IU/mL | ≥5 IU/mL | ≥50 IU/mL | N/A |
| Antigen source | Synthetic dsDNA |
Native calf thymus | Synthetic dsDNA | Purified native dsDNA |
Crithidia luciliae
substrate |
CIA: chemiluminescent immunoassay; ELISA: enzyme linked immunosorbent assay; ALBIA: addressable laser bead immunoassay; CLIFT: Crithidia luciliae indirect immunofluorescence test.