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. 2015 Feb 22;2015:495305. doi: 10.1155/2015/495305

Figure 2.

Figure 2

ROS were measured in MCF-7 cells treated for 2 h with juglone, Q7 or Q9 at 10 μM with or without ascorbate 1 mM (a). Integrity of PARP protein in MCF-7 cells treated with juglone (Jug), Q7 or Q9 at 20 μM with or without ascorbate (Asc) 1 mM for 24 h. Sanguinarine (Sang) 5 μM was used as a positive control of apoptosis (b). Colony-forming units of MCF-7 cells treated with juglone, Q7 or Q9 at 10 μM with/without ascorbate 1 mM for 2 h (c). Phosphorylated Akt (pAkt) was assessed by immunoelectrophoresis in MCF-7 cells treated for 24 h (d). Data were obtained from three independent experiments. (∗∗) and (∗∗∗) denote statistical differences at P < 0.01 and P < 0.001 compared to nontreated control cells or between indicated treatments, respectively.