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. 2015 Feb 5;2015:137981. doi: 10.1155/2015/137981

Figure 3.

Figure 3

Expression of TLR-4 receptor in purified splenic macrophages and lymphocytes after in vitro stimulation with LPS. The results in (a) and (c) show the differential translation of the TLR-4 mRNA to the protein in the splenic macrophages and lymphocytes, respectively, after in vitro stimulation with LPS from a set of triplicate experiments. Lane 1 (Mφ Con) represents TLR-4 on untreated macrophages, lane 2 (Mφ + L60 m) represents TLR-4 expression on the macrophages treated with LPS for 60 minutes, lane 3 (Mφ + L90 m) represents TLR-4 expression on macrophages treated with LPS for 90 minutes, and lane 4 (Mφ + 120 m) represents TLR-4 expression on macrophages treated with LPS for 120 minutes. Lane 1 (Lym Con) represents TLR-4 on untreated lymphocyte, lane 2 (Lym + L60 m) represents TLR-4 expression on the lymphocyte treated with LPS for 60 minutes, lane 3 (Lym + L90 m) represents TLR-4 expression on lymphocyte treated with LPS for 90 minutes, and lane 4 (Lym + L120 m) represents TLR-4 expression on lymphocyte treated with LPS for 120 minutes. The following symbols represent the significant fold change in TLR-4 expression between: *control versus LPS treated cells, #LPS treatment for 60 min versus LPS treatment for 90 and 120 min, %LPS treatment for 90 min versus LPS treatment for 120 min. Significant change (P < 0.05).