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. 2015 Feb 22;2015:505849. doi: 10.1155/2015/505849

Figure 3.

Figure 3

Insulin or the proteasomal inhibitor MG132 blocks SESN2 degradation. (a) HepG2 cells were pretreated with CHX for 30 min followed by a treatment with or without 100 nM of insulin (INS) for the indicated times. The cells were then lysed for SESN2 western blotting. Right panel is the plot of densitometric analysis. * P < 0.05 compared with noninsulin treatment. (b) and (c) HepG2 cells were treated with the indicated reagents for 30 min, followed by a treatment with or without 100 nM insulin for 8 h. The cells were then lysed for SESN2 western blotting. (d) HepG2 cells were incubated with or without insulin or MG132 for 18 h and the cells were lysed. Western blotting of SESN2 and β-actin (loading control) of total cellular proteins (left panel) or SESN2 immunoprecipitation followed by polyubiquitin antibody western blotting (right panel). IP indicates the immunoprecipitation antibody; IB indicates the immunoblotting antibody. Bottom panel of left panel: western blotting of the input samples. A representative blot of three independent experiments.