Table 2.
Top 20 most abundant clusters of transcripts present in leishmania exosomes
| L. donovani | L. braziliensis | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chr | Coordinates of genomic locus | Annotation | No. of reads | RNA biotype | Chr | Coordinates of genomic locus | Annotation | No. of reads | RNA biotype | ||
| 27 | 1014367 | 1019133 | LdBPK_27rRNA3 | 344191 | 28S rRNA | 6 | 334041 | 334897 | LmjF.27.rRNA.31 | 58906 | 28S rRNA |
| LdBPK_27rRNA4 | LmjF.27.rRNA.34 | ||||||||||
| LmjF.27.rRNA.13 | |||||||||||
| LmjF.27.rRNA.22 | |||||||||||
| LmjF.27.rRNA.29 | |||||||||||
| LmjF.27.rRNA.31 | |||||||||||
| LmjF.27.rRNA.33 | |||||||||||
| LmjF.27.rRNA.42 | |||||||||||
| 27 | 1019947 | 1021495 | LdBPK_27rRNA6 | 132730 | 18S rRNA | 00 | 463646 | 464099 | LbrM.27.rRNA1 | 11166 | 18S rRNA |
| 15 | 312758 | 313248 | LmjF.15.TRNAASP.01 | 65737 | tRNA-Asp | 15 | 324587 | 325394 | LbrM.15.tRNA1 | 18229 | tRNA-Asp |
| LmjF.15.TRNAGLU.01 | tRNA-Glu | LbrM.15.tRNA2 | tRNA-Glu | ||||||||
| LmjF.09.5SrRNA.02 | 5S rRNA | LbrM.15.rRNA1 | 5S rRNA | ||||||||
| LmjF.05.5SrRNA.01 | |||||||||||
| LmjF.15.5SrRNA.01 | |||||||||||
| 24 | 715730 | 715801 | LdBPK_24tRNA5 | 43207 | tRNA-Asp | 24 | 659346 | 659417 | LbrM.24.tRNA5 | 30583 | tRNA-Asp |
| 17 | 328838 | 328909 | LdBPK_17tRNA1 | 42601 | tRNA-Asp | 17 | 296223 | 296604 | LbrM.17.tRNA1 | 30523 | tRNA-Asp |
| LbrM.17.tRNA2 | tRNA-Ser | ||||||||||
| LbrM.17.tRNA3 | tRNA-Ala | ||||||||||
| 24 | 658796 | 658976 | LdBPK_24tRNA2 | 35611 | tRNA-Gln | 24 | 600448 | 600615 | LbrM.24.tRNA2 | 8749 | tRNA-Gln |
| 09 | 429809 | 430355 | LdBPK_09tRNA6 | 29246 | tRNA-Glu | 09 | 395278 | 395779 | LbrM.09.tRNA3 | 4467 | tRNA-Val |
| LmjF.09.TRNAARG.01 | tRNA-Arg | LbrM.09.tRNA4 | tRNA-His | ||||||||
| LmjF.09.TRNAVAL.02 | tRNA-Val | LbrM.09.rRNA1 | 5S rRNA | ||||||||
| LmjF.05.5SrRNA.01 | 5S rRNA | LmjF.05.5SrRNA.01 | |||||||||
| LmjF.11.5SrRNA.03 | LmjF.11.5SRRNA.03 | ||||||||||
| LmjF.21.5SrRNA.01 | LmjF.21.5SrRNA.02 | ||||||||||
| 09 | 403494 | 403565 | LbrM.09.tRNA5 | 5226 | tRNA-Glu | ||||||
| 31 | 495812 | 496115 | LdBPK_31tRNA3 | 18528 | tRNA-Glu | 31 | 582437 | 582738 | LbrM.31.tRNA2 | 4719 | tRNA-Gly |
| LbrM.31.tRNA3 | tRNA-Glu | ||||||||||
| 27 | 1019543 | 1019804 | LdBPK_27rRNA5 | 18473 | 5.8S rRNA | ||||||
| 11 | 156707 | 157038 | LmjF.11.TRNAALA.01 | 15493 | tRNA-Ala | 11 | 63421 | 63678 | LmjF.33.TRNAALA.01 | 3041 | tRNA-Ala |
| LmjF.36.TRNALEU.01 | tRNA-Leu | LmjF.11.TRNALEU.02 | tRNA-Leu | ||||||||
| 27 | 1014054 | 1014340 | LmjF.27.rRNA.47 | 14260 | 28S rRNA | ||||||
| LmjF.27.rRNA.48 | |||||||||||
| 23 | 230438 | 230509 | LdBPK_23tRNA9 | 13814 | tRNA-Gly | 23 | 216842 | 216916 | LbrM.23.tRNA9 | 3679 | tRNA-Gly |
| 36 | 1630332 | 1630403 | LdBPK_36tRNA2 | 11529 | tRNA-Gln | ||||||
| 05 | 360707 | 361335 | LdBPK_05snRNA1 | 9971 | snRNA | 05 | 349991 | 350587 | LbrM.05.rRNA1-1 | 6306 | 5S rRNA |
| LbrM.05.ncRNA1-1 | ncRNA | ||||||||||
| 33 | 104560 | 104930 | LdBPK_33tRNA1 | 9352 | tRNA-Ala | 33 | 105787 | 105859 | LbrM.33.tRNA1 | 5730 | tRNA-Arg |
| LdBPK_33tRNA2 | tRNA-Arg | ||||||||||
| LdBPK_33tRNA3 | |||||||||||
| 23 | 229645 | 229857 | LdBPK_23tRNA5 | 8487 | tRNA-Leu | 35 | 2472707 | 2472788 | LbrM.35.tRNA4 | 2592 | tRNA-Leu |
| LdBPK_23tRNA6 | tRNA-Thr | ||||||||||
| 16 | 445957 | 446028 | LdBPK_161tRNA1 | 7916 | tRNA-Gln | 16 | 442089 | 442160 | LbrM.16.tRNA1 | 8695 | tRNA-Gln |
| 23 | 230585 | 230656 | LdBPK_23tRNA10 | 7804 | tRNA-Trp | 23 | 216992 | 217063 | LbrM.23.tRNA10 | 3657 | tRNA-Trp |
| 21 | 430678 | 430798 | LbrM.21.rRNA1 | 3273 | 5S rRNA | ||||||
Reads were clustered into genomic loci based on Bowtie 2 alignments with reference genomes (as described in Methods) to identify the RNA biotypes that were most abundant in exosomes. The details of the top 20 clusters with the highest numbers of reads falling into them are listed. Clusters of reads in the L. donovani library are listed in descending order of abundance, with the homologous cluster of reads in the L. braziliensis library given in the same row. Chr = chromosome number, annotation = annotation in reference genomes (LdBPK = L. donovani, LbrM = L. braziliensis or LmjF = L. major) followed by the gene name, No. of reads = number of reads from the respective library falling into this cluster, RNA biotype = type(s) of RNA that is annotated in the reference genomes in this region.