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. 2015 Mar;43(3):418–423. doi: 10.1124/dmd.114.061887

Fig. 2.

Fig. 2.

The binding of EGCG to SULT1A1. Binding was monitored via ligand-induced changes in the intrinsic fluorescence of SULT1A1 (λex = 295 nm, λem = 345 nm). Conditions were as follows: SULT1A1 (10 nM, dimer), PAP (0 or 10 μM, 33 × Kd), pNP (0 or 45 μM, 30 × Kd), MgCl2 (5.0 mM), NaPO4 (50 mM), pH 7.2, T = 25 ± 2°C. Each point is the average of two independent determinations. The line through the data represents a least-squares fit using a model that assumes a single binding site per subunit.