Skip to main content
. 2015 Mar;352(3):519–528. doi: 10.1124/jpet.114.220350

Fig. 2.

Fig. 2.

Relative contributions of cation-selective transporters to the AP uptake of metformin into Caco-2 cell monolayers determined using a novel chemical inhibition scheme. Inhibition of metformin uptake (10 µM, 5 minutes) into OCT1-, 2-, and 3-expressing CHO cells by mitoxantrone (A), corticosterone (B), cimetidine (C), and desipramine (D). Data represent mean ± S.D., n = 3. Inhibition curves were fit to corrected uptake rate in the presence of varying concentrations of each inhibitor. (E) Chemical inhibition scheme to determine the contributions of transporters to metformin AP uptake in Caco-2 cell monolayers. (F) Inhibition of metformin AP uptake (10 µM, 5 minutes) in Caco-2 cell monolayers in the presence of chemical inhibitors shown in (E). Data represent mean ± S.D., n = 3. **P < 0.01, ***P < 0.001 compared with the control; #P < 0.05 compared with each other.