Fig. 2. The effect of TvSP on the cytokine production of BMDC. DCs were differentiated with GM-CSF from BM precursors in the absence or presence of TvSP. (A-E) Immature DCs were stimulated with 100 ng/ml LPS for 6 h, and the mRNA levels of Il12a (A), Il12b (B), Il10 (C), Tnf (D), and Il6 (E) were measured by qRT-PCR. (F-I) Immature DCs were stimulated with 100 ng/ml LPS for 24 h, and the expression of IL-12 (F), IL-10 (G), TNF-α (H), and IL-6 (I) was measured by Cytometric Bead Array. All data are expressed as mean ± SD, and statistical significance (*P < 0.05, or **P < 0.01) is shown for treatments compared with the controls.