Fig. 4. TvSP exhibit epigenetic control via modulation of histone modifications at the promoter region of the Il12a gene. DCs were differentiated with GM-CSF from BM precursors in the absence or presence of TvSP. On day 10, LPS (100 ng/ml) was added to the culture for 1 h and H3K4me3 levels at the promoter regions of Il12a (A), Il12b (B), Il10 (C), Tnf (D), Il6 (E) and Gapdh (F) genes were analyzed by ChIP assay. *P< 0.05 compared with untreated cells. Results are representative of more than three independent experiments.
