FIG 3.
Measuring the TPP-binding affinity of TP_0144 and TDE_0143. (a) SDS-PAGE analysis of purified recombinant TP_0144 (rTP_0144) and TDE_0143 (rTDE_0143) proteins and their mutated versions (rTP_0144227Y/A and rTDE_0143227Y/A). For each protein, lanes 1 and 2 represent uninduced and induced E. coli lysates, respectively; lane 3 for each protein represents the purified recombinant protein. (b and c) Plots of equilibrium binding of the recombinant proteins and TPP. Equilibrium dialysis was performed as previously described (21). The binding constants were determined with GraphPad Prism, version 5 (one site, specific binding).