TABLE 1.
Oligonucleotide primers used in this study
| Primer | Sequencea | Orientationb | Description and/or purpose |
|---|---|---|---|
| P1 | CACCGCTCACCGTATGCGTGTT | F | TP_0144 recombinant protein |
| P2 | TCATGGGGTTGATGCGTG | R | TP_0144 recombinant protein |
| P3 | GCCGCACTGGTACTTAGTGCCACCACAAGTGC | F | Site mutagenesis of TP_0144 |
| P4 | GCACTTGTGGTGGCACTAAGTACCAGTGCGGC | R | Site mutagenesis of TP_0144 |
| P5 | GCTCCCATAGCCATTTCTGCCACAAGCAGCC | F | Site mutagenesis of TDE_0143 |
| P6 | GGCTGCTTGTGGCAGAAATGGCTATGGGAGC | R | Site mutagenesis of TDE_0143 |
| P7 | CGCCACCTAAGGACAACTTGTTATTATGCAC | F | Complementation, PtbpA |
| P8 | CATACGGTGAGCCATATGCTCCCTCCGCCG | R | Complementation, PtbpA |
| P9 | GGGAGCATATGGCTCACCGTATGCGTGTTGTCTTC | F | Complementation, TP_0144 |
| P10 | ACTAGTTCATGGGGTTGATGCGTGTAATGC | R | Complementation, TP_0144 |
| P11 | ACTAGTCATCGTTCATATTATACTTCTCC | F | Complementation, Ptap1-aacCm |
| P12 | CAAGTTGTCCTTAGGTGGCGGTACTTGGGTC | R | Complementation, Ptap1-aacCm |
| P13 | ACTAGTATGAAAAATCATTTACGCTC | F | Complementation, TDE_0143 |
| P14 | GAACGATGTTATTTTTGTAAAACATTGATTACGGC | R | Complementation, TDE_0143 |
| P15 | CAATGTTTTACAAAAATAACATCGTTCATATTATATTCTCC | F | Complementation, Ptap1-aacCm |
| P16 | GCGGCCGCTTAGGTGGCGGTACTTGGGTC | R | Complementation, Ptap1-aacCm |
| P17 | CGGTTGTGCCGCTCTTTTTGCAG | F | TDE_0143 DNA probe |
| P18 | GCAAAATAGCCGTAGTCAAAGGG | R | TDE_0143 DNA probe |
| P19 | CTCACCGTATGCGTGTTGTC | F | TP_0144 DNA probe |
| P20 | CGGATGGCACGATGAATAGC | R | TP_0144 DNA probe |
a
The engineered restriction enzyme sites are underlined.
b
F, forward; R, reverse.