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Effect of nonphosphorylated PEP analogs on L. plantarum Xfp and C. neoformans Xfp2 activities. Reactions were performed using L. plantarum Xfp (A) and C. neoformans Xfp2 (B) F6P and Pi K0.5 substrate concentrations. Concentrations of 8 mM (dark gray) and 16 mM (light gray) for each nonphosphorylated PEP analog were tested in triplicate. Activity is reported as a percentage of activity with no ligand present.
