TABLE 1.
Enzyme and substrate | K0.5 (mM) | kcat app (s−1) | kcat app/K0.5 (s−1 mM−1) | H |
---|---|---|---|---|
L. plantarum Xfp | ||||
F6P | 11.0 ± 1.4 | 1.05 ± 0.05 | 0.10 ± 0.01 | 0.99 ± 0.04 |
Pi | 1.0 ± 0.1 | 1.12 ± 0.03 | 1.11 ± 0.09 | 0.68 ± 0.02 |
C. neoformans Xfp2a | ||||
F6P | 15.9 ± 1.3 | 3.47 ± 0.10 | 0.22 ± 0.01 | 1.41 ± 0.11 |
Pi | 13.3 ± 1.5 | 4.22 ± 0.13 | 0.32 ± 0.03 | 0.59 ± 0.03 |
Previously reported kinetic parameters (6).