FIG 4.
SepD/SepL-dependent secretion signals in translocators EspA and EspD. (A) Schematic diagrams of EspA, EspB, and EspD. (B) Type III secretion assay of TEM-1 fusions to different EspA truncations. Whole bacterial lysates (WCL) and secreted proteins (SP) were analyzed by Western blotting using antibodies against TEM-1. (C) Type III secretion assay of TEM-1 fusions to different EspD truncations. Whole bacterial lysates (WCL) and secreted proteins (SP) were analyzed by Western blotting using antibodies against TEM-1.