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Expression of the VapB4 (Rv0596c)-myc-6× His or VapB4-sfGFP fusion in trans inhibits the toxicity of VapC4 (Rv0595c). (A) The open reading frame of VapB4 from M. tuberculosis was cloned into the expression plasmid pJSB31 or pJSB31-sfGFP, creating a myc-6× His fusion or a sfGFP fusion, respectively. (B) The growth of E. coli strains carrying the indicated plasmids was compared using the serial dilution cell spotting assay, as described in Materials and Methods.
