TABLE 1.

VapB4 Δ54 antitoxin loss-of-function mutations

Type	Mutations
Single	D82G, L61F, T66A, T66P, L68Q, D81G, T80S, T80K, L61T, L68P, Q78R, I55V, T80K, D82N, T66S, D81A, Q78R, D64N, T80M, G62S, D64E, E71A, E74V, D64E, V59E, D64N, T79A, I55V
Double	T80P, R84Q; R60H, D64V; R73C, T75S; N67D, T79A; E74G, T75S; T66I, T75S; T65N, L76Q; N67D, V83A; T75I, L68P; D64G, L76Q; P63L, E71G; Q78H, T84G; Q78R, D82G; R60H, D81G; R60C, N67Y
Triple	E70D, Q78R, T80S; I55F, L76Q, T77M; P63H, L72P, D82E; G56C, T79I, V83A