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. 2015 Feb 17;112(9):2817–2822. doi: 10.1073/pnas.1414055112

Fig. 5.

Fig. 5.

SK channel is required for ionophore-mediated NETosis. (A) Human neutrophils were activated with A23187 in the presence of SK channel inhibitor NS8593 (100 µM). The plate reader assay shows that NETosis is significantly reduced in the presence of NS8593 (n = 3), suggesting that A23187-induced NET DNA release in human neutrophils requires the activation of the SK channel. (B) Neutrophils were activated with PMA in the presence of NS8593. The plate reader assays show that NETosis is not inhibited in the presence of NS8593 (100 µM; n = 3), indicating that PMA-mediated NETosis does not require the activation of the SK channel. (C and D) Human neutrophils were activated with A23187 (C) or ionomycin (D) in the presence of SK channel inhibitor apamin (200 nM; n = 3). The plate reader assays show that NETosis is reduced significantly in the presence of apamin; hence, A23187- and ionomycin-induced NETosis requires the activation of the SK channel. A23, A23187; Io, ionomycin. *P < 0.05; **P < 0.01; ***P < 0.001 compared with the agonist alone (two-way ANOVA).