Fig. 2.

Dendritic cell SIRT1 alters the differentiation of CD4⁺T-cell lineage against microbial-induced inflammation. (A) The protein level of SIRT1 in the DC cells following stimulation with LPS (10 ng/mL), TNFα (100 ng/mL), IL-12 (10 ng/mL), IFN-γ (50 ng/mL), IL-10 (20 ng/mL) and TGF-β1 (5 ng/mL). (B and C) SIRT1^CD11c−/− mice were infected with L. monocytogenes. The intracellular staining of IFN-γ, IL-17A and Foxp3 in the spleen was determined following LLO_189–201 stimulation at day 7. (D–F) T cells (CD4⁺TCR⁺CD45Rb^hiCD25⁻) were transferred into Rag1^−/− and SIRT1^CD11c−/−Rag1^−/− mice, and body weight was measured weekly (D) and representative colon histology (E). The intracellular staining of IFN-γ, IL-17A and Foxp3 in donor cells (CD45.1⁺ cells) isolated from the MLNs (F). Data (B–F) are shown as mean ± SD, from one of four (B–F) independent experiments (at least four mice per group). **P < 0.01 and ***P < 0.001 compared with the indicated groups.