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. 2015 Feb 17;112(9):E957–E965. doi: 10.1073/pnas.1420419112

Fig. 4.

Fig. 4.

DC–SIRT1 signaling directs TH1 and Treg differentiation in vitro. (A and B) Naive OT-II T cells were cocultured with WT or SIRT1CD11c−/− DCs in the presence of antigen for 5 d. (A) Intracellular staining of IFN-γ and Foxp3 expression in cultured T cells. (Right) The proportions of IFN-γ+ and Foxp3+ proportion in CD4+ T cells. (B) The mRNA expression of indicated genes in T cells restimulated with α-CD3 for 5 h. (C and D) Naive T cells from C57BL/6 mice were cocultured with WT or SIRT1CD11c−/− DCs in the presence of α-CD3 for 5 d. (C) The proportions of IFN-γ+ and Foxp3+ proportion in CD4+ T cells. (D) The mRNA expression of indicated genes in OT-II T cells. Data are representative of two (A and B) and three (C and D) independent experiments (three to five mice per group), **P < 0.01 and ***P < 0.001 compared with the indicated groups.