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. 2015 Feb 17;112(9):E957–E965. doi: 10.1073/pnas.1420419112

Fig. 5.

Fig. 5.

SIRT1-dependent IL-12 and TGF-β1 production in DCs regulates TH1 and Treg cell differentiation. (A) The intracellular staining of indicated genes in WT and SIRT1CD11c−/− splenic DCs 5 h following LPS stimulation in vitro. (Right) The proportion of IL-12p40+ and intracellular TGF-β1 expression (mean fluorescence intensity, MFI) among CD11c+ cells. (B and C) The mRNA expression of IL-12p70 and TGF-β1 in WT and SIRT1CD11c−/− splenic DCs following LPS stimulation in vitro (B) and in vivo (C) were detected. (D) The intracellular staining of IFN-γ and Foxp3 in T cells cocultured with WT or SIRT1CD11c−/− splenic DCs with indicated treatment. (Right) The proportion of IFN-γ+ and Foxp3+ proportion in CD4+ T cells. Data are representative of four independent experiments (three to five mice per group). *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the indicated groups.