Table 1. Polyclonal irisin antibodies used in this and related ELISA kits.
| Designation (this study) | Manufacturer/Vendor (Cat. #) | Antigen (epitope)1 | Manufacturer (ELISA #) | Range of ELISA2 | Remarks |
|---|---|---|---|---|---|
| pAb-A | Aviscera/Phoenix (G-067-52) | recombinant irisin (aa 33–142) | Phoenix (EK-067-52) | 0.3–205 ng/mL | antibody discontinued; ELISA replaced by EK-067-29 |
| pAb-B | Cayman (14625) | synthetic irisin (aa 33–142) | - | - | no ELISA available |
| pAb-C | Adipogen (A-25B-0027) | recombinant irisin (aa 33–142) | Adipogen (AG-45A-0046EK) | 1.0–5,000 ng/mL | antibody is epitope affinity purified |
| pAb-D | Phoenix (G-067-16) | synthetic irisin (aa 33–142) | Phoenix (EK-067-16) | 0.1–1,000 ng/mL | ELISA not used in this study |
| pAb-E | BioVision/BioCat(AP8746b-AB) | C-terminus of FNDC5 (aa 149–178) | - | - | no ELISA available antibody is epitope affinity purified |
1refers to GenPept accession number NP_715637.2.
2data of manufacturer/vendor.