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. 2015 Mar 9;5:8864. doi: 10.1038/srep08864

Figure 2. The inhibitory effect of five natural compounds on LLO activity.

Figure 2

(a) Purified rLLO pretreated with the indicated concentrations of natural compounds was incubated with defibrinated sheep erythrocytes and hemolysis activity was determined by the release of hemoglobin at OD543. (b) Hemolysis assay was performed as described above to examine the inhibitory effects of myricetin on the hemolysis induced by LLO and its mutants. Bars represent the standard deviation (*P < 0.05, **P < 0.01; two tailed Student's t-test).