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. 2015 Mar 9;5:8854. doi: 10.1038/srep08854

Figure 3. (P) RR is essential for activating the Wnt/β-catenin signaling pathway in human PDAC cell lines.

Figure 3

(a) Representative image of Wnt3a expression among three human PDAC cell lines. Consistent results were observed when three experiments were repeated. (b) Wnt3a (150 ng/mL) significantly increased LRP6 activity in PK-1 cells (mean ± SEM, n = 3 for each). *P < 0.05 vs. vehicle-treated cells. Blotting with an anti-LRP6 antibody showed equal loading. (c) Wnt3a significantly increased active β-catenin expression in PK-1 cells (mean ± SEM, n = 3 for each). *P < 0.05 vs. vehicle-treated cells. β-actin was used as a loading control. (d) Effect of (P)RR siRNA on LRP6 activity in Wnt3a-treated PK-1 cells (mean ± SEM, n = 3 for each). Blotting with an anti-LRP6 antibody showed equal loading. (P)RR protein expression indicates efficient gene transfection. (e) Effect of (P)RR siRNA on active β-catenin and Cyclin D1 expression in PK-1, BxPC-3 and PANC-1 cells (mean ± SEM, n = 3 for each, *P < 0.05 vs. active β-catenin expression in scrambled siRNA-transfected cells; **P < 0.05 vs. active β-catenin expression in scrambled siRNA-transfected cells stimulated with Wnt3a; #P < 0.05 vs. CyclinD1 expression in scrambled siRNA-transfected cells; §P < 0.05 vs. Cyclin D1 expression of scrambled siRNA-transfected cells stimulated with Wnt3a). β-actin was used as a loading control.