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. 2015 Mar 9;5:8854. doi: 10.1038/srep08854

Figure 5. Wild-type β-catenin or constitutively active mutant β-catenin constructs were cotransfected into PK-1 cells with scrambled siRNA or (P)RR siRNA expression.

Figure 5

(a) Effect of wild-type β-catenin (wt β-catenin) on the (P)RR siRNA-induced reduction in PK-1 cell proliferative ability. (b) The protein expression of (P)RR and Myc indicates efficiency of gene transfection with (P)RR (ATP6AP2) siRNA and a plasmid carrying a constitutively active aa 1–90-deleted β-catenin tagged with Myc, respectively. (c) Effect of constitutively active mutant β-catenin (mut β-catenin) on (P)RR siRNA-induced reduction in PK-1 cell proliferative ability. Cell proliferation was evaluated at 24 h and 48 h after Wnt 3a stimulation by WST-1 assays (mean ± SEM, n = 3 for each, P < 0.05 vs. (P)RR siRNA cells). N.S., not significant.