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. 2015 Mar 9;5:8854. doi: 10.1038/srep08854

Figure 6. Loss of (P)RR function induces apoptosis of human PDAC cells.

Figure 6

(a) Distributions of the populations of scrambled siRNA- and (P)RR siRNA-treated cells after 48 h of Wnt3a stimulation. Experiments were repeated in triplicate, leading to the similar results. FS: Forward Scatter; SS: Side Scatter. (b) Cell cycle analyses of scrambled siRNA- and (P)RR siRNA-treated cells stimulated with or without Wnt3a. Cells were labeled with PI and analyzed by DNA flow cytometry. The arrow shows a sub-G1 apoptotic peak. (c) Cell cycle distribution of PK-1 (left) and PANC-1 (right) cells expressing scrambled siRNA and (P)RR siRNA after 48 h of Wnt3a stimulation. (d) Effect of (P)RR siRNA on caspase-3 activity in PK-1, BxPC-3 and PANC-1 cell lines after 48 h of Wnt3a stimulation (mean ± SEM, n = 3 for each, *P < 0.05 vs. scrambled siRNA cells). (P)RR siRNA dramatically increased caspase-3 activity in all three cell lines.